Within AAV and lentiviral production QC is needed to determine the viral loads. Nest to traditional techniques such as analytical ultracentrifugation (AUC), ELISA and digital-PCR, Mass photometry is a new powerful technology.

Mass photometry provides a label-free, single-particle resolution analysis that allows AAV and lentiviral vectors to be directly characterized by mass, without the need for extensive sample preparation. The technology accurately distinguishes between empty, partially filled and fully filled capsids, as well as aggregate detection, allowing for real-time monitoring of essential quality parameters with minimal material input. This positions mass photometry as a powerful, fast and cost-effective QC solution to replace or complement traditional techniques such as analytical ultracentrifugation (AUC), ELISA and digital-PCR. All technologies will be explained including hands-on experience in this course